Expression of a bacterial mtlD gene in transgenic tobacco leads to production and accumulation of mannitol.

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Expression of a bacterial mtlD gene in transgenic tobacco leads to production and accumulation of mannitol.

A bacterial gene encoding mannitol-1-phosphate dehydrogenase, mtlD, was engineered for expression in higher plants. Gene constructions were stably incorporated into tobacco plants. The mtlD gene was expressed and translated into a functional enzyme in tobacco, resulting in the synthesis and accumulation of mannitol, which was identified by NMR and mass spectroscopy. Mannitol concentrations exce...

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ژورنال

عنوان ژورنال: Proceedings of the National Academy of Sciences

سال: 1992

ISSN: 0027-8424,1091-6490

DOI: 10.1073/pnas.89.7.2600